Botryoccocus braunii Triterpene Synthase Proteins and Nucleic Acid Molecules, and Methods for Their Use

ABSTRACT

This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.

CLAIM TO PRIORITY

This application is a divisional of U.S. application Ser. No.12/539,442, filed Aug. 11, 2009 which claims priority under 35 U.S.C.119(e) to U.S. provisional application No. 61/087,920 filed Aug. 11,2008, incorporated in its entirety by reference.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has beensubmitted via EFS-Web and is hereby incorporated by reference in itsentirety. Said ASCII copy, created on Oct. 12, 2009, is named14726673.txt, and is 14,577 bytes in size.

FIELD OF THE INVENTION

This invention relates to a triterpene synthase, in particular abotryococcene synthase, proteins and nucleic acid molecules and theiruse.

BACKGROUND OF THE INVENTION

Squalene and botryococcene are related by their putative biosyntheticorigins from the condensation of two farnesyl diphosphate (FPP)molecules, and are known to be synthesized byrace B, a fresh water greenalgae (Okada. et al., (1995). Journal Of Applied Phycology 555-559;Metzger and Largeau (2005). Applied Microbiology and Biotechnology486-496.) Botryococcene is further modified in and becomes methylatedwith 1, 2, 3 or 4 additional methyl substituents catalyzed by a specialtriterpene methyl transferase. Botryococene and its methylatedderivatives have attracted significant attention because these moleculesare thought to be the progenitors to current oil shale deposits (Summonset al., (2002) Organic Geochemistry 99-109; Walters et al., (2005) AapgBulletin 1239-1 244.) and because they are considered promisingrenewable, alternative biofuels (Banerjee et al., (2002). CriticalReviews in Biotechnology 245-279.) For example, Hillen et al. (1982)Biotechnology And Bioengineering 193-205) previously reported on thecatalytic cracking of methylated botryococcenes and squalenederivatives, and observed an overall conversion of 97% of the oil tocombustible fuels under standard cracking conditions. Overall, 67% ofthe oil was converted to gasoline grade fuel, 15% to aviation turbinefuel, and 15% to diesel fuel with a residual of only 3%. Hence,catalytic hydrolysis (as performed in standard petroleum refineries) ofthese highly branched, poly-unsaturated triterpenes results in thegeneration of hydrocarbon fractions that are chemically equivalent tothose derived from current petroleum deposits and are of direct utilityas fuels for internal combustion engines, as well as feedstocks forchemical manufacturing (Banerjee et al., (2002)).

Up to this time, these energy-rich triterpene oils have only beenavailable from cultures of a rather slow growing green algae that doesnot lend itself to large-scale or fermentation type culturing conditions(Casadevall et al., (1985). Biotechnology and Bioengineering 286-295).

BRIEF DESCRIPTION OF THE INVENTION

The current disclosure describes the functional identification andcharacterization of the gene coding for a triterpene synthase inparticular a botryococcene synthase, BBS, enzyme. The identification ofthe botryococcene synthase gene now provides an alternative means ofgenerating important raw materials for the reliable and cost effectiveproduction of an energy-rich, renewable, and sustainable biofuel source(FIG. 3). For example, the co-expression of the botryococcene synthasegene in combination with a suitable FPP synthase and triterpenemethyltransferase genes in transgenic terrestrial or aquatic plantscould yield a production platform for the methylated triterpenes. Thesecompounds could be derived from the metabolic diversion of CO₂ fixed inthe process of photosynthesis flowing directly into triterpenebiosynthesis and accumulation. The feasibility of this engineeringstrategy for the production of large amounts of high-valuedsesquiterpenes, terpenes consisting of 15 carbons rather than the 30carbons found in triterpenes, was recently demonstrated. (Wu et al.,(2006) Nature Biotechnology 14411447).

The botryococcene synthase of this invention is a triterpene synthaseenzyme catalyzing the reductive condensation of 2 farnesyl diphosphate(FPP) substrate molecules yielding botryococcene, a 30-carbon,branched-chain hydrocarbon.

More specifically, disclosed herein is the DNA and protein sequence of,and the functional characterization for, the race B botryococcenesynthase gene which, when expressed in a heterologous host such asbacteria, yeast or plants yields a protein that, when mixed with alysate and reducing equivalents in the form of NADPH, provides an enzymeactivity that catalyzes a unique chemical condensation of 2 FPPmolecules, creating a branched, triterpene hydrocarbon known asbotryococcene. A schematic diagram of the reaction catalyzed by thebotryococcene synthase enzyme is shown in FIG. 1. Like squalene synthase(Blagg et al., (2002) J. Am. Chem. Soc. 8846-8853), botryococcenesynthase is predicted to catalyze a 2 step reaction. The first stepcondenses the 2 FPP substrate molecules into a pre-squalene diphosphateintermediate (PSPP). When NADPH is provided, squalene synthase reducesthe PSPP to a linkage of carbons 1 and 1′ of the respective FPPmolecules. In contrast, when botryococcene synthase is provided NADPH,the PSPP undergoes a different reducing rearrangement to botryococcenewith a linkage of carbon 3 of one FPP starter molecule to carbon 1′ ofthe second FPP starter molecule.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts the biosynthetic pathways for botryococcene and squalene.Both triterpenes are derived from an initial condensation of 2 FPPmolecules to form presqualene diphosphate (PSPP), which is subsequentlycleaved and reduced to form either botryococcene or squalene.

FIG. 2 depicts an overview of the hydrocracking process of a typical,C₃₄ botryococcene to yield fuel suitable for combustion engines.Briefly, botryococcocenes are treated with high pressure H₂ at hightemperatures with a Pd catalyst to give a variety of organic molecules,which can be further distilled into various classes of fuel.

FIG. 3 depicts a strategy for engineering an alternative biofuelsproduction platform into terrestrial or aquatic plants. Genes coding forthe biochemical steps for synthesis of botryococcocenes would beintroduced into plants such that the respective catalytic steps (FPPsynthase, triterpene synthase and triterpene methyltransferaseactivities) would be targeted to the chloroplast compartment. Theintroduced enzyme activities would hence divert photosynthetically fixedCO₂ directly into methylated triterpene biosynthesis.

FIG. 4 presents the DNA sequence for the botryococcene synthase cDNA,SEQ ID NO: 1. The start and stop codons are shown in bold.

FIG. 5 presents the amino acid sequence for the botryococcene synthaseprotein, SEQ ID NO: 2, predicted from the corresponding cDNA sequenceshown in FIG. 4.

FIG. 6 depicts an alignment comparison of selection regions/domains ofvarious squalene synthase proteins from Botryococcus (BSS), tobacco (N.tabacum), Arabidopsis (A. thaliana), corn (Z. [[maize]]mays), rat (R.rattus) and yeast (S. cerevisiae) to that for the botryococcene synthase(BBS). Domains I-VI were identified as highly conserved amongst diversesqualene synthases, and domains III-V were previously correlated withthe particular steps in catalysis as noted. Sequences displayed acrossthe 5 domains are: BBS, SEQ ID NO: 3, 4, 5, 6, and 7; BSS, SEQ ID NO: 8,9, 10, 11, and 12; N. tabacum SEQ ID NO: 13, 14, 15, 16, and 17; A.thaliana, SEQ ID NO: 18, 19, 20, 21, and 22; Z. [[maize]]mays, SEQ IDNO: 23, 24, 25, 26 and 27; R. rattus, SEQ ID NO: 28, 29, 30, 31, ans 32;and S. cerevisiae SEQ ID NO: 33, 34, 35, 36 and 37.

FIG. 7 depicts the purification of hexa-histidine tagged (SEQ ID NO: 44)BBS enzyme. E. coli cells over-expressing the botryococcene synthasegene harboring an amino terminal hexa-histidine purification tag (SEQ IDNO: 44) were used to prepare an initial cell lysate (crude lysate),which was then subject to nickle affinity chromatography. The crudelysate was applied to the affinity column, followed by washing withbuffer containing increasing concentrations of imidazole (flow (bufferwithout imidazole, to buffer containing 250 mM imidazole) and therespective column fractions collected for SDS-PAGE analysis. Aliquots ofeach fraction were resolved by SDS-PAGE and the gel stained withCoomassie Blue, a general protein stain. Molecular weight standards arenoted as marker and the expected size for the his-tagged BBS protein isnoted by the arrow.

FIG. 8 illustrates the botryococcene synthase enzyme assay.

FIG. 9 depicts the enzyme activity of purified BBS protein, activityassociated with a B. braunii lysate (boty lys), and BBS combined withlysate (BBS+boty lys) with or without NADPH. BBS containing anN-terminal hexa-histidine tag (SEQ ID NO: 44) was expressed in E. coliand purified from bacterial lysate by nickel affinity chromatographyaccording to the manufacturer (Novagen) (see FIG. 7).

FIG. 10 depicts GC chromatographs of hexane extracts prepared from TN7yeast (a) and TN7 yeast expressing BBS (b). The mass spectrum for theunique peak found in the TN7-BBS culture with a retention time of 16.20is shown in panel c. The mass spectrum matches that of presqualenealcohol (PSOH) as described by Edmond et al. (1971) J. Biol. Chem.6254-6251.

FIG. 11 depicts GC chromatographs of hexane extracts prepared from TN7yeast (a) and TN7 yeast expressing a full length B. braunii squalenesynthase gene (b). Identification of the novel compound accumulating inTN7 yeast expressing the squalene synthase gene (corresponding to thepeak with retention time of approximately 14) was based on mass spectralcomparisons (not shown) and identical chromatographic behavior of anauthentic squalene (25 ng) standard (c).

FIG. 12 depicts the accumulation of squalene in TN7 yeastover-expressing a Botryococcus braunii squalene synthase gene.

DETAILED DESCRIPTION OF THE INVENTION

The current disclosure describes the functional identification andcharacterization of the gene coding for a botryococcene synthase, BBS,enzyme, nucleic acid molecules e.g, SEQ ID NO: 1, encoding abotryococcene synthase, BBS, polypeptide of this invention, e.g., SEQ IDNO: 2 and methods for their use.

The polypeptides of this invention include for example polypeptidescomprising the amino acid sequence set forth in SEQ ID NO:2 andfragments thereof. Preferably the polypeptide fragments have triterpenesynthase activity. The polypeptides of this invention may comprise oneor more peptide domains I, II, III, IV, V and IV, wherein domain Icomprises LPQELQDPICIFYL (SEQ ID NO: 3.), domain II comprisesLRALDTVEDDMN LKSETK (SEQ ID NO; 4), domain III comprises YCHYVAGSCGIAVTKVIV (SEQ ID NO: 5), domain IV comprises GLLLQKANIITD YNED (SEQ ID NO6), and domain V comprises ALALLLVTAFGHLS (SEQ ID NO: 7).

The polypeptides of this invention may also contain one or more modifiedamino acids. The presence of modified amino acids may be advantageousin, for example, increasing triterpene synthase catalytic activity orincreasing polypeptide stability. Amino acid(s) are modified, forexample, co-translationally or post-translationally during recombinantproduction (e.g., N-linked glycosylation at N—X—S/T motifs duringexpression in mammalian cells) or modified by synthetic means.Accordingly, a “mutant”, “variant” or “modified” protein, enzyme,polynucleotide, gene, or cell, means a protein, enzyme, polynucleotide,gene, or cell, that has been altered or derived, or is in some waydifferent or changed, from a parent protein, enzyme, polynucleotide,gene, or cell. A mutant or modified protein or enzyme is usually,although not necessarily, expressed from a mutant polynucleotide orgene.

A “parent” protein, enzyme, polynucleotide, gene, or cell, is anyprotein, enzyme, polynucleotide, gene, or cell, from which any otherprotein, enzyme, polynucleotide, gene, or cell, is derived or made,using any methods, tools or techniques, and whether or not the parent isitself native or mutant. A parent polynucleotide or gene encodes for aparent protein or enzyme.

A “mutation” means any process or mechanism resulting in a mutantprotein, enzyme, polynucleotide, gene, or cell. This includes anymutation in which a protein, enzyme, polynucleotide, or gene sequence isaltered, and any detectable change in a cell arising from such amutation. Typically, a mutation occurs in a polynucleotide or genesequence, by point mutations, deletions, or insertions of single ormultiple nucleotide residues. A mutation includes polynucleotidealterations arising within a protein-encoding region of a gene as wellas alterations in regions outside of a protein-encoding sequence, suchas, but not limited to, regulatory or promoter sequences. A mutation ina gene can be “silent”, i.e., not reflected in an amino acid alterationupon expression, leading to a “sequence-conservative” variant of thegene. This generally arises because of degeneracy of the genetic codewherein more than one codon codes for the same amino acid.

Non-limiting examples of a modified amino acid include a glycosylatedamino acid, a sulfated amino acid, a prenlyated (e.g., farnesylated,geranylgeranylated) amino acid, an acetylated amino acid, an acylatedamino acid, a pegylated amino acid, a biotinylated amino acid, acarboxylated amino acid, a phosphorylated amino acid, and the like.References adequate to guide one of skill in the modification of aminoacids are replete throughout the literature. Example protocols are foundin Walker (1998) Protein Protocols on CD-ROM (Humana Press, Towata,N.J.).

Recombinant methods for producing and isolating the triterpene synthasepolypeptides and modified triterpene synthase polypeptides of theinvention are described herein. In addition to recombinant production,the polypeptides may be produced by direct peptide synthesis usingsolid-phase techniques (e.g., Stewart et al. (1969) Solid-Phase PeptideSynthesis (WH Freeman Co, San Francisco); and Merrifield (1963) J. Am.Chem. Soc. 85: 2149-2154; each of which is incorporated by reference).Peptide synthesis may be performed using manual techniques or byautomation. Automated synthesis may be achieved, for example, usingApplied Biosystems 431A Peptide Synthesizer (Perkin Elmer, Foster City,Calif.) in accordance with the instructions provided by themanufacturer.

A “protein” or “polypeptide”, which terms are used interchangeablyherein, comprises one or more chains of chemical building blocks calledamino acids that are linked together by chemical bonds called peptidebonds. An “enzyme” means any substance, composed wholly or largely ofprotein, that catalyzes or promotes, more or less specifically, one ormore chemical or biochemical reactions. A “native” or “wild-type”protein, enzyme, polynucleotide, gene, or cell, means a protein, enzyme,polynucleotide, gene, or cell that occurs in nature (whose formpredominates in natural populations).

Accordingly, in various embodiments, isolated or recombinantpolypeptides comprising the amino acid sequence set forth in SEQ ID NO:2are provided. The polypeptides include up to 35, 25, 10, 5, 4, 3, 2 or 1conservative amino acid substitutions.

“Conservative amino acid substitutions” or, simply, “conservativevariations” of a particular sequence refers to the replacement of oneamino acid, or series of amino acids, with essentially identical aminoacid or series of amino acids. One of skill will recognize thatindividual substitutions, deletions or additions which alter, add ordelete a single amino acid or a percentage of amino acids in an encodedsequence result in “conservative variations” where the alterationsresult in the deletion of an amino acid, addition of an amino acid, orsubstitution of an amino acid with a functionally similar amino acid.

Conservative substitution tables providing functionally similar aminoacids are well known in the art. For example, one conservativesubstitution group includes Alanine (A), Serine (S), and Threonine (T).Another conservative substitution group includes Aspartic acid (D) andGlutamic acid (E). Another conservative substitution group includesAsparagine (N) and Glutamine (Q). Yet another conservative substitutiongroup includes Arginine (R) and Lysine (K). Another conservativesubstitution group includes Isoleucine, (I) Leucine (L), Methionine (M),and Valine (V). Another conservative substitution group includesPhenylalanine (F), Tyrosine (Y), and Tryptophan (W).

Thus, “conservative amino acid substitutions” of a listed polypeptidesequence (e.g., SEQ ID NO:2) include substitutions of a percentage,typically less than 10%, of the amino acids of the polypeptide sequence,with an amino acid of the same conservative substitution group.Accordingly, a conservatively substituted variation of a polypeptide ofthe invention can contain, for example, substitutions of 35, 25, 10, 5,4, 3, 2 or 1 amino acid with an amino acid of the same conservativesubstitution group.

It is understood that the addition of sequences that do not alter theencoded activity of a nucleic acid molecule, such as the addition of anon-functional or non-coding sequence, is a conservative variation ofthe basic nucleic acid molecule. The “activity” of an enzyme is ameasure of its ability to catalyze a reaction, i.e., to “function”, andmay be expressed as the rate at which the product of the reaction isproduced. For example, enzyme activity can be represented as the amountof product produced per unit of time or per unit of enzyme (e.g.,concentration or weight), or in terms of affinity or dissociationconstants. As used interchangeably herein a “triterpene synthaseactivity”, “biological activity of triterpene synthase” or “functionalactivity of triterpene synthase”, refers to an activity exerted by atriterpene synthase protein, polypeptide or nucleic acid molecule on atriterpene synthase polypeptide substrate, as determined in vivo, or invitro, according to standard techniques.

One of skill in the art will appreciate that many conservativesubstitutions of the nucleic acid constructs which are disclosed hereinyield a functionally identical construct. For example, owing to thedegeneracy of the genetic code, “silent substitutions” (i.e.,substitutions in a nucleic acid sequence which do not result in analteration in an encoded polypeptide) are an implied feature of everynucleic acid sequence which encodes an amino acid.

Similarly, “conservative amino acid substitutions,” in which one or afew amino acids in an amino acid sequence are substituted with differentamino acids with highly similar properties, are also readily identifiedas being highly similar to a disclosed construct. Families of amino acidresidues having similar side chains have been defined in the art. Thesefamilies include amino acids with basic side chains (e.g., lysine,arginine, histidine), acidic side chains (e.g., aspartic acid, glutamicacid), uncharged polar side chains (e.g., glycine, asparagine,glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains(e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine,methionine, tryptophan), beta-branched side chains (e.g., threonine,valine, isoleucine) and aromatic side chains (e.g., tyrosine,phenylalanine, tryptophan, histidine). Such conservative variations ofeach disclosed sequence are a feature of the polypeptides providedherein.

It will be appreciated by those skilled in the art that due to thedegeneracy of the genetic code, a multitude of nucleotide sequencesencoding modified triterpene synthase polypeptides of the invention maybe produced, some of which bear substantial identity to the nucleic acidsequences explicitly disclosed herein. For instance, codons AGA, AGG,CGA, CGC, CGG, and CGU all encode the amino acid arginine. Thus, atevery position in the nucleic acid molecules of the invention where anarginine is specified by a codon, the codon can be altered to any of thecorresponding codons described above without altering the encodedpolypeptide. It is understood that U in an RNA sequence corresponds to Tin a DNA sequence.

“Conservative variants” are proteins or enzymes in which a given aminoacid residue has been changed without altering overall conformation andfunction of the protein or enzyme, including, but not limited to,replacement of an amino acid with one having similar properties,including polar or non-polar character, size, shape and charge. Aminoacids other than those indicated as conserved may differ in a protein orenzyme so that the percent protein or amino acid sequence similaritybetween any two proteins of similar function may vary and can be, forexample, at least 30%, at least 50%, at least 70%, at least 80%, atleast 90%, at least 95%, at least 98% or at least 99%, as determinedaccording to an alignment scheme. As referred to herein, “sequencesimilarity” means the extent to which nucleotide or protein sequencesare related. The extent of similarity between two sequences can be basedon percent sequence identity and/or conservation. “Sequence identity”herein means the extent to which two nucleotide or amino acid sequencesare invariant. “Sequence alignment” means the process of lining up twoor more sequences to achieve maximal levels of identity (and, in thecase of amino acid sequences, conservation) for the purpose of assessingthe degree of similarity. Numerous methods for aligning sequences andassessing similarity/identity are known in the art such as, for example,the Cluster Method, wherein similarity is based on the MEGALIGNalgorithm, as well as BLASTN, BLASTP, and FASTA (Lipman and Pearson,(1985) Science 22; 227(4693):1435-41; Pearson and Lipman, (1988) ProcNatl Acad Sci USA; 85(8):2444-8). When using all of these programs, thepreferred settings are those that results in the highest sequencesimilarity.

Non-conservative modifications of a particular polypeptide are thosewhich substitute any amino acid not characterized as a conservativesubstitution. For example, any substitution which crosses the bounds ofthe six groups set forth above. These include substitutions of basic oracidic amino acids for neutral amino acids, (e.g., Asp, Glu, Asn, or Glnfor Val, Ile, Leu or Met), aromatic amino acid for basic or acidic aminoacids (e.g., Phe, Tyr or Trp for Asp, Asn, Glu or Gln) or any othersubstitution not replacing an amino acid with a like amino acid. Basicamino acids include lysine (K), arginine (R), histidine (H); acidicamino acids include aspartic acid (D), glutamic acid (E); unchargedpolar amino acids include glycine (G), asparagine (N), glutamine (Q),serine (S), threonine (T), tyrosine (Y), cysteine (C); nonpolar aminoacids include alanine (A), valine (V), leucine (L), isoleucine (I),proline (P), phenylalanine (F), methionine (M), tryptophan (W);beta-branched amino acids include threonine (T), valine (V), isoleucine(I); aromatic amino acids include tyrosine (Y), phenylalanine (F),tryptophan (W), histidine (H).

A polynucleotide, polypeptide, or other component is “isolated” when itis partially or completely separated from components with which it isnormally associated (other proteins, nucleic acid molecules, cells,synthetic reagents, etc.). A nucleic acid molecule or polypeptide is“recombinant” when it is artificial or engineered, or derived from anartificial or engineered protein or nucleic acid molecule. For example,a polynucleotide that is inserted into a vector or any otherheterologous location, e.g., in a genome of a recombinant organism, suchthat it is not associated with nucleotide sequences that normally flankthe polynucleotide as it is found in nature is a recombinantpolynucleotide. A protein expressed in vitro or in vivo from arecombinant polynucleotide is an example of a recombinant polypeptide.Likewise, a polynucleotide sequence that does not appear in nature, forexample a variant of a naturally occurring gene, is recombinant. Forexample, an “isolated” nucleic acid molecule is one which is separatedfrom other nucleic acid molecules which are present in the naturalsource of the nucleic acid molecule. For example, with regards togenomic DNA, the term “isolated” includes nucleic acid molecules whichare separated from the chromosome with which the genomic DNA isnaturally associated. Typically, an “isolated” nucleic acid molecule isfree of sequences which naturally flank the nucleic acid molecule (i.e.,sequences located at the 5′ and 3′ ends of the nucleic acid molecule) inthe genomic DNA of the organism from which the nucleic acid molecule isderived. For example, in various embodiments, the isolated nucleic acidmolecule can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5kb or 0.1 kb of nucleotide sequences which naturally flank the nucleicacid molecule in genomic DNA of the cell from which the nucleic acidmolecule is derived. Moreover, an “isolated” nucleic acid molecule, suchas a cDNA molecule, can be substantially free of other cellularmaterial, or culture medium when produced by recombinant techniques, orsubstantially free of chemical precursors or other chemicals whenchemically synthesized.

In some embodiments, a polypeptide provided herein includes amino acidresidue substitutions that correspond to positions in a particularsequence at least 80%, 85%, 90%, 95%, 98% or 99% of the time. In otherwords, the invention encompasses polypeptides that contain the recitedamino acid substitutions at 80%, 85%, 90%, 95%, 98% or 99% of therecited positions in a given sequence. The skilled artisan willrecognize that not every substitution from a group of substitutions isnecessary to obtain a modified polypeptide that is active on atriterpene substrate:

“Sequence identity” herein means the extent to which two nucleotide oramino acid sequences are invariant. “Sequence alignment” means theprocess of lining up two or more sequences to achieve maximal levels ofidentity (and, in the case of amino acid sequences, conservation) forthe purpose of assessing the degree of similarity. Numerous methods foraligning sequences and assessing similarity/identity are known in theart such as, for example, the Cluster Method, wherein similarity isbased on the MEGALIGN algorithm, as well as BLASTN, BLASTP, and FASTA(Lipman and Pearson, (1985), Pearson and Lipman, (1988)). When using allof these programs, the preferred settings are those that results in thehighest sequence similarity. For example, the “identity” or “percentidentity” with respect to a particular pair of aligned amino acidsequences can refer to the percent amino acid sequence identity that isobtained by ClustalW analysis (version W 1.8 available from EuropeanBioinformatics Institute, Cambridge, UK), counting the number ofidentical matches in the alignment and dividing such number of identicalmatches by the greater of (i) the length of the aligned sequences, and(ii) 96, and using the following default ClustalW parameters to achieveslow/accurate pairwise alignments—Gap Open Penalty: 10; Gap ExtensionPenalty: 0.10; Protein weight matrix: Gonnet series; DNA weight matrix:IUB; Toggle Slow/Fast pairwise alignments=SLOW or FULL Alignment.

Two sequences are “optimally aligned” when they are aligned forsimilarity scoring using a defined amino acid substitution matrix (e.g.,BLOSUM62), gap existence penalty and gap extension penalty so as toarrive at the highest score possible for that pair of sequences. Aminoacid substitution matrices and their use in quantifying the similaritybetween two sequences are well-known in the art and described, e.g., inDayhoff et al. (1978) “A model of evolutionary change in proteins” in“Atlas of Protein Sequence and Structure,” Vol. 5, Suppl. 3 (ed. M. O.Dayhoff), pp. 345-352. Natl. Biomed. Res. Found., Washington, D.C. andHenikoff et al. (1992) Proc. Nat'l. Acad. Sci. USA 89: 10915-10919 (eachof which is incorporated by reference). The BLOSUM62 matrix is oftenused as a default scoring substitution matrix in sequence alignmentprotocols such as Gapped BLAST 2.0. The gap existence penalty is imposedfor the introduction of a single amino acid gap in one of the alignedsequences, and the gap extension penalty is imposed for each additionalempty amino acid position inserted into an already opened gap. Thealignment is defined by the amino acids positions of each sequence atwhich the alignment begins and ends, and optionally by the insertion ofa gap or multiple gaps in one or both sequences so as to arrive at thehighest possible score. While optimal alignment and scoring can beaccomplished manually, the process is facilitated by the use of acomputer-implemented alignment algorithm, e.g., gapped BLAST 2.0,described in Altschul et al. (1997) Nucl. Acids Res. 25: 3389-3402(incorporated by reference herein), and made available to the public atthe National Center for Biotechnology Information (NCBI) Website(www.ncbi.nlm.nih.gov). Optimal alignments, including multiplealignments, can be prepared using, e.g., PSI-BLAST, available throughthe NCB1 website and described by Altschul et al. (1997) Nucl. AcidsRes. 25:3389-3402 (incorporated by reference herein).

With respect to an amino acid sequence that is optimally aligned with areference sequence, an amino acid residue “corresponds to” the positionin the reference sequence with which the residue is paired in thealignment. The “position” is denoted by a number that sequentiallyidentifies each amino acid in the reference sequence based on itsposition relative to the N-terminus. For example, in SEQ ID NO:2,position 1 is M, position 2 is T, position 3 is M, etc. When a testsequence is optimally aligned with SEQ ID NO:2, a residue in the testsequence that aligns with the M at position 3 is said to “correspond toposition 3” of SEQ ID NO:2. Owing to deletions, insertion, truncations,fusions, etc., that must be taken into account when determining anoptimal alignment, in general the amino acid residue number in a testsequence as determined by simply counting from the N-terminal will notnecessarily be the same as the number of its corresponding position inthe reference sequence. For example, in a case where there is a deletionin an aligned test sequence, there will be no amino acid thatcorresponds to a position in the reference sequence at the site ofdeletion. Where there is an insertion in an aligned reference sequence,that insertion will not correspond to any amino acid position in thereference sequence. In the case of truncations or fusions there can bestretches of amino acids in either the reference or aligned sequencethat do not correspond to any amino acid in the corresponding sequence.

Also contemplated are fragments of the full length triterpene synthasepolypeptides and polynucleotides, e.g., fragments of polypeptidescomprising the amino acid sequence set forth in SEQ ID NO: 2 andfragments of nucleic acid molecules comprising the sequence set forth inSEQ ID NO: 1. A “fragment” is a unique portion of a triterpene synthasepolypeptide or the polynucleotide encoding triterpene synthase which isidentical in sequence to, but shorter in length than, the parentsequence. A fragment may comprise up to the entire length of the definedsequence, minus one nucleotide/amino acid residue. For example, afragment may comprise from 5 to 1000 contiguous nucleotides or aminoacid residues of a given nucleic acid molecule or polypeptide. Afragment used as a probe, primer, antigen, catalytic molecule, or forother purposes, may be at least 5, 10, 15, 16, 20, 25, 30, 40, 50, 60,75, 100, 150, 250 or at least 500 contiguous nucleotides or amino acidresidues in length. Fragments may be preferentially selected fromcertain regions of a molecule. For example, a polypeptide fragment maycomprise a certain length of contiguous amino acids selected from thefirst 250 or 500 amino acids (or first 25% or 50%) of a polypeptide asshown in a certain defined sequence. Clearly these lengths areexemplary, and any length that is supported by the specification,including the Sequence Listing, tables, and figures, may be encompassedby the present embodiments.

Also contemplated in this invention are isolated polypeptides that aretriterpene synthases, that comprise 5 peptide domains I, II, III, IV,and V. The peptide domains I, II, III, IV, and V of the triterpenesynthase of this invention may comprise respectively, e.g., SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7 (See FIG.6, the 5 domains of B. braunii (BBS)). In an embodiment of theinvention, domain I comprises an amino acid sequence that is at least20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95% identical to thefull-length of SEQ ID NO: 3, domain II comprises an amino acid sequencethat is at least 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95% identicalthe full-length of SEQ ID NO: 4, domain III may comprise an amino acidsequence that is at least 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 95%identical to the full-length of SEQ ID NO: 5, domain IV comprises anamino acid sequence that is at least 20%, 30%, 40%, 50%, 60%, 70%, 80%,90% or 95% identical to the full-length of SEQ ID NO: 6 and domain Vcomprises an amino acid sequence that is at least 20%, 305, 40%, 50%,60%, 70%, 80%, 90% or 95% identical the full-length of SEQ ID NO: 7. The5 peptide domains may be present in the synthase in any order,preferably the order of the 5 peptides in the polypeptide is, from itsamino to carboxy terminal, I, II, III, IV, and V, and more preferablythe I, II, III, IV and V domains comprise respectively SEQ ID NO: 3, SEQID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7.

In other embodiments, isolated nucleic acid molecules are provided.Described herein are nucleic acid molecules that encode a polypeptidehaving triterpene synthase activity, in particular a Botryococcusbraunii triterpene synthase. The nucleic acid molecules of thisinvention include e.g., nucleic acid molecules that encode the aminoacid sequence set forth in SEQ ID NO: 2, nucleic acid molecules thatencode fragments of SEQ ID NO: 2, nucleic acid molecules that compriseSEQ ID NO:1, and nucleic acid molecules that encode fragments of SEQ IDNO: 1. In one aspect, the invention provides a novel family of isolatedor recombinant polynucleotides referred to herein as “triterpenesynthase polynucleotides” or “triterpene synthase nucleic acidmolecules.” Triterpene synthase polynucleotide sequences arecharacterized by the ability to encode a triterpene synthasepolypeptide. In general, the invention includes any nucleotide sequencethat encodes any of the novel triterpene synthase polypeptides describedherein. The terms “polynucleotide,” “nucleotide sequence,” and “nucleicacid molecule” are used to refer to a polymer of nucleotides (A, C, T,U, G, etc. or naturally occurring or artificial nucleotide analogues),e.g., DNA or RNA, or a representation thereof, e.g., a character string,etc., depending on the relevant context. A given polynucleotide orcomplementary polynucleotide can be determined from any specifiednucleotide sequence.

In an aspect, the triterpene synthase polynucleotides compriserecombinant or isolated forms of naturally occurring nucleic acidmolecules isolated from an organism, e.g., an algae strain. Exemplarytriterpene synthase polynucleotides include those that encode thepolypeptide set forth in SEQ ID NO:2. In another aspect of theinvention, triterpene synthase polynucleotides are produced bydiversifying, e.g., mutating, a naturally occurring, isolated, orrecombinant triterpene synthase polynucleotide, e.g., the nucleic acidsequence set forth in SEQ ID NO: 1. It is possible to generatediversified triterpene synthase polynucleotides encoding triterpenesynthase polypeptides with superior functional attributes, e.g.,increased catalytic function, increased stability, or higher expressionlevel, than a triterpene synthase encoded by the polynucleotide used asa substrate or parent in the diversification process.

The polynucleotides of the invention have a variety of uses in, forexample recombinant production (i.e., expression) of the triterpenesynthase polypeptides of the invention and as substrates for furtherdiversity generation, e.g., recombination reactions or mutationreactions to produce new and/or improved triterpene synthase homologues,and the like.

It is important to note that certain specific, substantial and credibleutilities of triterpene synthase polynucleotides do not require that thepolynucleotide encode a polypeptide with substantial triterpene synthaseactivity or even variant triterpene synthase activity. For example,triterpene synthase polynucleotides that do not encode active enzymescan be valuable sources of parental polynucleotides for use indiversification procedures to arrive at triterpene synthasepolynucleotide variants, or non-triterpene synthase polynucleotides,with desirable functional properties (e.g., high k_(cat) ork_(cat)/K_(m), low K_(m), high stability towards heat or otherenvironmental factors, high transcription or translation rates,resistance to proteolytic cleavage, etc.).

Triterpene synthase polynucleotides, including nucleotide sequences thatencode triterpene synthase polypeptides and variants thereof, fragmentsof triterpene synthase polypeptides, related fusion proteins, orfunctional equivalents thereof, are used in recombinant DNA moleculesthat direct the expression of the triterpene synthase polypeptides inappropriate host cells, such as plant cells. Due to the inherentdegeneracy of the genetic code, other nucleic acid sequences whichencode substantially the same or a functionally equivalent amino acidsequence can also be used to clone and express the triterpene synthasepolynucleotides. The term “host cell”, as used herein, includes any celltype which is susceptible to transformation with a nucleic acidconstruct. The term “transformation” means the introduction of a foreign(i.e., extrinsic or extracellular) gene, DNA or RNA sequence to a hostcell, so that the host cell will express the introduced gene or sequenceto produce a desired substance, typically a protein or enzyme coded bythe introduced gene or sequence. The introduced gene or sequence mayinclude regulatory or control sequences, such as start, stop, promoter,signal, secretion, or other sequences used by the genetic machinery ofthe cell. A host cell that receives and expresses introduced DNA or RNAhas been “transformed” and is a “transformant” or a “clone.” The DNA orRNA introduced to a host cell can come from any source, including cellsof the same genus or species as the host cell, or cells of a differentgenus or species.

As will be understood by those of skill in the art, it can beadvantageous to modify a coding sequence to enhance its expression in aparticular host. The genetic code is redundant with 64 possible codons,but most organisms preferentially use a subset of these codons. Thecodons that are utilized most often in a species are called optimalcodons, and those not utilized very often are classified as rare orlow-usage codons (see, e.g., Zhang et al. (1991) Gene 105:61-72;incorporated by reference herein). Codons can be substituted to reflectthe preferred codon usage of the host, a process sometimes called “codonoptimization” or “controlling for species codon bias.”

Optimized coding sequences containing codons preferred by a particularprokaryotic or eukaryotic host (see also, Murray et al. (1989) Nucl.Acids Res. 17:477-508; incorporated by reference herein) can beprepared, for example, to increase the rate of translation or to producerecombinant RNA transcripts having desirable properties, such as alonger half-life, as compared with transcripts produced from anon-optimized sequence. Translation stop codons can also be modified toreflect host preference. For example, preferred stop codons for S.cerevisiae and mammals are UAA and UGA, respectively. The preferred stopcodon for monocotyledonous plants is UGA, whereas insects and E. coliprefer to use UAA as the stop codon (Dalphin et al. (1996) Nucl. AcidsRes. 24: 216-218; incorporated by reference herein). Methodology foroptimizing a nucleotide sequence for expression in a plant is provided,for example, in U.S. Pat. No. 6,015,891, and the references citedtherein (incorporated herein by reference).

“Silent variations” are one species of “conservative substitutions.” Oneof skill will recognize that each codon in a nucleic acid sequence(except AUG, which is ordinarily the only codon for methionine) can bemodified by standard techniques to encode a functionally identicalpolypeptide. Accordingly, each silent variation of a nucleic acidsequence that encodes a polypeptide is implicit in any describedsequence. The invention provides each and every possible variation ofnucleic acid sequence encoding a polypeptide of the invention that couldbe made by selecting combinations based on possible codon choices. Thesecombinations are made in accordance with the standard triplet geneticcode as applied to the nucleic acid sequence encoding a triterpenesynthase homologue polypeptide of the invention. All such variations ofevery nucleic acid sequence herein are specifically provided anddescribed by consideration of the sequence in combination with thegenetic code. Any variant can be produced as noted herein.

In general, the invention includes any polypeptide encoded by a modifiedtriterpene synthase polynucleotide derived by mutation, recursivesequence recombination, and/or diversification of the polynucleotidesequences described herein. In some aspects of the invention, atriterpene synthase polypeptide is modified by single or multiple aminoacid substitutions, a deletion, an insertion, or a combination of one ormore of these types of modifications. Substitutions can be conservativeor non-conservative, can alter function or not, and can add newfunction. Insertions and deletions can be substantial, such as the caseof a truncation of a substantial fragment of the sequence, or in thefusion of additional sequence, either internally or at N or C terminal.

An aspect of the invention pertains to isolated nucleic acid moleculesthat encode modified triterpene synthase polypeptides or biologicallyactive portions thereof. As used herein, the term “nucleic acidmolecule” is intended to include DNA molecules (e.g., cDNA or genomicDNA) and RNA molecules (e.g., mRNA) and analogs of the DNA or RNAgenerated using nucleotide analogs. The nucleic acid molecule can besingle-stranded or double-stranded, but preferably is double-strandedDNA.

A nucleic acid molecule of the present invention, e.g., a nucleic acidmolecule that encodes a polypeptide set forth in SEQ ID NO:2, or havingthe nucleotide sequence of set forth in SEQ ID NO:1, or a portionthereof, can be isolated using standard molecular biology techniques andthe sequence information provided herein.

A nucleic acid molecule of the invention can be amplified using cDNA,mRNA or alternatively, genomic DNA, as a template and appropriateoligonucleotide primers according to standard PCR amplificationtechniques. The nucleic acid molecule so amplified can be cloned into anappropriate vector and characterized by DNA sequence analysis.Furthermore, oligonucleotides corresponding to nucleotide sequences canbe prepared by standard synthetic techniques, e.g., using an automatedDNA synthesizer. In some embodiments, an isolated nucleic acid moleculeof the invention comprises a nucleic acid molecule which is a complementof a nucleotide sequence encoding a polypeptide set forth in SEQ IDNO:2, or complement of the nucleotide sequence set forth in SEQ ID NO:1.In still another embodiment, an isolated nucleic acid molecule of theinvention comprises a nucleotide sequence which is at least about 50%,52%, 55%, 60%, 62%, 65%, 70%, 75%, 78%, 80%, 85%, 88%, 90%, 95%, 97%,98% or more identical to the nucleotide sequence encoding a polypeptideset forth in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ IDNO:1, or a portion of any of these nucleotide sequences.

In addition to the nucleotide sequences encoding a polypeptide set forthin SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, itwill be appreciated by those skilled in the art that DNA sequencepolymorphisms that lead to changes in the amino acid sequences of theproteins may exist within a population. Such genetic polymorphisms mayexist among individuals within a population due to natural allelicvariation. Such natural allelic variations include both functional andnon-functional proteins and can typically result in 1-5% variance in thenucleotide sequence of a gene. Any and all such nucleotide variationsand resulting amino acid polymorphisms in genes that are the result ofnatural allelic variation and that do not alter the functional activityof a protein are intended to be within the scope of the invention.

Accordingly, in another embodiment, an isolated nucleic acid molecule ofthe invention hybridizes under stringent conditions to a nucleic acidmolecule comprising the nucleotide sequence encoding a polypeptide setforth in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ IDNO:1. In other embodiments, the nucleic acid molecule is at least 30,50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, or 600 nucleotidesin length. Nucleic acid molecules are “hybridizable” to each other whenat least one strand of one polynucleotide can anneal to anotherpolynucleotide under defined stringency conditions. Stringency ofhybridization is determined, e.g., by (a) the temperature at whichhybridization and/or washing is performed, and (b) the ionic strengthand polarity (e.g., formamide) of the hybridization and washingsolutions, as well as other parameters. Hybridization requires that thetwo polynucleotides contain substantially complementary sequences;depending on the stringency of hybridization, however, mismatches may betolerated. Typically, hybridization of two sequences at high stringency(such as, for example, in an aqueous solution of 0.5×SSC at 65° C.)requires that the sequences exhibit some high degree of complementarityover their entire sequence. Conditions of intermediate stringency (suchas, for example, an aqueous solution of 2×SSC at 65° C.) and lowstringency (such as, for example, an aqueous solution of 2×SSC at 55°C.), require correspondingly less overall complementarity between thehybridizing sequences (1×SSC is 0.15 M NaCl, 0.015 M Na citrate).

Nucleic acid molecules that hybridize include those which anneal undersuitable stringency conditions and which encode polypeptides or enzymeshaving the same function, such as the ability to catalyze the reductivecondensation of 2 farnexyl diphosphate (FPP) substrate moleculesyielding botryococcene, a 30-carbon branched-chain hydrocarbon, of theinvention. Further, the term “hybridizes under stringent conditions” isintended to describe conditions for hybridization and washing underwhich nucleotide sequences at least 30%, 40%, 50%, or 60% homologous toeach other typically remain hybridized to each other. Preferably, theconditions are such that sequences at least about 70%, more preferablyat least about 80%, even more preferably at least about 85% or 90%homologous to each other typically remain hybridized to each other. Insome cases, an isolated nucleic acid molecule of the invention thathybridizes under stringent conditions to a nucleic acid sequenceencoding a polypeptide set forth in any of SEQ ID NO:2, or thenucleotide sequence set forth in SEQ ID NO:1, corresponds to anaturally-occurring nucleic acid molecule. As used herein, a“naturally-occurring” nucleic acid molecule refers to an RNA or DNAmolecule having a nucleotide sequence that occurs in nature (e.g.,encodes a natural protein). Preferably the nucleic acid molecule thathybridizes, hybridizes to at least 30%, 40%, 50%, 60%, 70%, 80%, 85% or90% of the length of a nucleic acid molecule consisting of SEQ ID NO:1under stringent conditions. More preferably the nucleic acid moleculethat hybridizes, hybridizes to at least about 80%, even more preferablyat least about 85% or 90% of the length of a nucleic acid moleculeconsisting of SEQ ID NO: 1. Preferably the nucleic acid molecule thathybridizes encodes a polypeptide having triterpene synthase activity.

The skilled artisan will appreciate that changes can be introduced bymutation into the nucleotide sequences of any nucleic acid sequenceencoding a polypeptide set forth in SEQ ID NO:2, or having thenucleotide sequence set forth in SEQ ID NO:1, thereby leading to changesin the amino acid sequence of the encoded proteins. In some cases thealteration will lead to altered function of the polypeptide. In othercases the change will not alter the functional ability of the encodedpolypeptide. In general, substitutions that do not alter the function ofa polypeptide include nucleotide substitutions leading to amino acidsubstitutions at “non-essential” amino acid residues. Generally thesesubstitutions can be made in, for example, the sequence encoding apolypeptide set forth in SEQ ID NO:2, or having the nucleotide sequenceset forth in SEQ ID NO:1, without altering the ability of the enzyme tocatalyze the reductive condensation of FPP substrate. A “non-essential”amino acid residue is a residue that can be altered from the parentsequence without altering the biological activity of the resultingpolypeptide, e.g., catalyzing the reductive condensation of 2 FPP toyield botryococcene.

Also contemplated are those situations where it is desirable to alterthe activity of a parent polypeptide such that the polypeptide has newor increased activity on a particular substrate. It is understood thatthese amino acid substitutions will generally not constitute“conservative” substitutions. Instead, these substitutions constitutenon-conservative substitutions introduced in to a sequence in order toobtain a new or improved activity.

It is also understood that an isolated nucleic acid molecule encoding apolypeptide homologous to the polypeptide of SEQ ID NO:2 can be createdby introducing one or more nucleotide substitutions, additions ordeletions into the nucleotide sequence encoding the particularpolypeptide, such that one or more amino acid substitutions, additionsor deletions are introduced into the encoded protein. Mutations can beintroduced into the nucleic acid sequence by standard techniques, suchas site-directed mutagenesis and PCR-mediated mutagenesis. In contrastto those positions where it may be desirable to make a non-conservativeamino acid substitutions (see above), in some positions it is preferableto make conservative amino acid substitutions.

Mutational methods of generating diversity include, for example,site-directed mutagenesis (Ling et al. (1997) Anal Brioche. 254(2):157-178; Dale et al. (1996) Methods Mol. Biol. 57:369-374; Smith (1985)Ann. Rev. Genet. 19:423-462; Botstein & Shortle (1985) Science229:1193-1201; Carter (1986) Biochem. J. 237:1-7; and Kunkel (1987) “Theefficiency of oligonucleotide directed mutagenesis” in Nucleic Acids &Molecular Biology (Eckstein, F. and Lilley, D. M. J. eds., SpringerVerlag, Berlin)); mutagenesis using uracil containing templates (Kunkel(1985) Proc. Natl. Acad. Sci. USA 82:488-492; Kunkel et al. (1987)Methods in Enzymol. 154, 367-382; and Bass et al. (1988) Science242:240-245); oligonucleotide-directed mutagenesis (Methods in Enzymol.100: 468-500 (1983); Methods in Enzymol. 154: 329-350 (1987); Zoller &Smith (1982) Nucleic Acids Res. 10:6487-6500; Zoller & Smith (1983)Methods in Enzymol. 100:468-500; and Zoller & Smith (1987) Methods inEnzymol. 154:329-350); phosphorothioate-modified DNA mutagenesis (Tayloret al. (1985) Nucl. Acids Res. 13: 8749-8764; Taylor et al. (1985) Nucl.Acids Res. 13: 8765-8787; Nakamaye & Eckstein (1986) Nucl. Acids Res.14: 9679-9698; Sayers et al. (1988) Nucl. Acids Res. 16:791-802; andSayers et al. (1988) Nucl. Acids Res. 16: 803-814); mutagenesis usinggapped duplex DNA (Kramer et al. (1984) Nucl. Acids Res. 12: 9441-9456;Kramer & Fritz (1987) Methods in Enzymol. 154:350-367; Kramer et al.(1988) Nucl. Acids Res. 16: 7207; and Fritz et al. (1988) Nucl. AcidsRes. 16: 6987-6999) (each of which is incorporated by reference).

Additional suitable methods include point mismatch repair (Kramer et al.(1984) Cell 38:879-887), mutagenesis using repair-deficient host strains(Carter et al. (1985) Nucl. Acids Res. 13: 4431-4443; and Carter (1987)Methods in Enzymol. 154: 382-403), deletion mutagenesis (Eghtedarzadeh &Henikoff (1986) Nucl. Acids Res. 14: 5115), restriction-selection andrestriction-purification (Wells et al. (1986) Phil. Trans. R. Soc. Lond.A 317: 415-423), mutagenesis by total gene synthesis (Nambiar et al.(1984) Science 223: 1299-1301; Sakamar and Khorana (1988) Nucl. AcidsRes. 14: 6361-6372; Wells et al. (1985) Gene 34:315-323; and Grundstromet al. (1985) Nucl. Acids Res. 13: 3305-3316); double-strand breakrepair (Mandecki (1986); Arnold (1993) Current Opinion in Biotechnology4:450-455; and Proc. Natl. Acad. Sci. USA, 83:7177-7181) (each of whichis incorporated by reference). Additional details on many of the abovemethods can be found in Methods in Enzymology (1987) Volume 154, whichalso describes useful controls for trouble-shooting problems withvarious mutagenesis methods.

Additional details regarding various diversity generating methods can befound in the following U.S. patents, PCT publications, and EPOpublications: U.S. Pat. No. 5,605,793 to Stemmer (Feb. 25, 1997); U.S.Pat. No. 5,811,238 to Stemmer et al. (Sep. 22, 1998); U.S. Pat. No.5,830,721 to Stemmer et al. (Nov. 3, 1998); U.S. Pat. No. 5,834,252 toStemmer, et al. (Nov. 10, 1998); U.S. Pat. No. 5,837,458 to Minshull, etal. (Nov. 17, 1998); WO 95/22625, Stemmer and Crameri; WO 96/33207 byStemmer and Lipschutz; WO 97/20078 by Stemmer and Crameri; WO 97/35966by Minshull and Stemmer; WO 99/41402 by Punnonen et al.; WO 99/41383 byPunnonen et al.; WO 99/41369 by Punnonen et al.; WO 99/41368 by Punnonenet al.; EP 752008 by Stemmer and Crameri; EP 0932670 by Stemmer; WO99/23107 by Stemmer et al.; WO 99/21979 by Apt et al.; WO 98/31837 bydel Cardayre et al.; WO 98/27230 by Patten and Stemmer; WO 98/13487 byStemmer et al.; WO 00/00632; WO 00/09679; WO 98/42832 by Arnold et al.;WO 99/29902 by Arnold et al.; WO 98/41653 by Vind; WO 98/41622 byBorchert et al.; WO 98/42727 by Pati and Zarling; WO 00/18906 by Pattenet al.; WO 00/04190 by del Cardayre et al.; WO 00/42561 by Crameri etal.; WO 00/42559 by Selifonov and Stemmer; WO 00/42560 by Selifonov etal.; WO 01/23401 by Welch et al.; and WO 01/64864 by Affholter (each ofwhich is incorporated by reference). The QUICKCHANGE™ protocol marketedby Stratagene of San Diego, Calif. is one specific method known to thoseskilled in the art for introducing site-directed mutations. This methodrelies on the use of oligo or DNA primer pairs, harboring specific DNAsequence changes to be introduced, annealed to the target DNA or gene tobe modified. Copies of modified DNA/gene are amplified by standard PCRmethodology. Confirmation of alteration of the target DNA sequence isverifiable by automated DNA sequencing.

Also provided are recombinant constructs comprising one or more of thenucleic acid sequences as broadly described above. The constructscomprise a vector, such as, a plasmid, a cosmid, a phage, a virus, abacterial artificial chromosome (BAC), a yeast artificial chromosome(YAC), or the like, into which a nucleic acid sequence of the inventionhas been inserted, in a forward or reverse orientation. In a preferredaspect of this embodiment, the construct further comprises regulatorysequences including, for example, a promoter operably linked to thesequence. Large numbers of suitable vectors and promoters are known tothose of skill in the art, and are commercially available.

Accordingly, in other embodiments, vectors that include a nucleic acidmolecule of the invention are provided.

In other embodiments, host cells transfected with a nucleic acidmolecule of the invention, or a vector that includes a nucleic acidmolecule of the invention, are provided. Host cells include eucaryoticcells such as yeast cells, e.g., yeast cells having a ERG1 knockout,e.g., the yeast strain TN7 described in co-pending application Ser. No.12/489,038 incorporated herein in its entirety, insect cells, animalcells, or plant cells (e.g., algal cells or terrestrial plant cells).Host cells also include procaryotic cells such as bacterial cells.

The terms “vector”, “vector construct” and “expression vector” mean thevehicle by which a DNA or RNA sequence (e.g. a foreign gene) can beintroduced into a host cell, so as to transform the host and promoteexpression (e.g. transcription and translation) of the introducedsequence. Vectors typically comprise the DNA of a transmissible agent,into which foreign DNA encoding a protein is inserted by restrictionenzyme technology. A common type of vector is a “plasmid”, whichgenerally is a self-contained molecule of double-stranded DNA that canreadily accept additional (foreign) DNA and which can readily introducedinto a suitable host cell. A large number of vectors, including plasmidand viral vectors, have been described for replication and/or expressionin a variety of eukaryotic and prokaryotic hosts. Non-limiting examplesinclude pKK plasmids (Clonetech), pUC plasmids, pET plasmids (Novagen,Inc., Madison, Wis.), pRSET or pREP plasmids (Invitrogen, San Diego,Calif.), pMAL plasmids (New England Biolabs, Beverly, Mass.), and Tiplasmid vectors, and many appropriate host cells, using methodsdisclosed or cited herein or otherwise known to those skilled in therelevant art. Vectors can also be selected such that expression of theintroduced sequence is targeted to a chloroplast in a plant cell.Recombinant cloning vectors will often include one or more replicationsystems for cloning or expression, one or more markers for selection inthe host, e.g., antibiotic resistance, and one or more expressioncassettes.

The terms “express” and “expression” mean allowing or causing theinformation in a gene or DNA sequence to become manifest, for exampleproducing a protein by activating the cellular functions involved intranscription and translation of a corresponding gene or DNA sequence. ADNA sequence is expressed in or by a cell to form an “expressionproduct” such as a protein. The expression product itself, e.g. theresulting protein, may also be said to be “expressed” by the cell. Apolynucleotide or polypeptide is expressed recombinantly, for example,when it is expressed or produced in a foreign host cell under thecontrol of a foreign or native promoter, or in a native host cell underthe control of a foreign promoter.

Polynucleotides provided herein can be incorporated into any one of avariety of expression vectors suitable for expressing a polypeptide.Suitable vectors include chromosomal, nonchromosomal and synthetic DNAsequences, e.g., derivatives of SV40; bacterial plasmids; phage DNA;baculovirus; yeast plasmids; vectors derived from combinations ofplasmids and phage DNA, viral DNA such as vaccinia, adenovirus, fowl poxvirus, pseudorabies, adenovirus, adeno-associated viruses, retroviruses;Ti plasmids for the incorporation and expression of DNA in plant cells,and many others. Any vector that transduces genetic material into acell, and, if replication is desired, which is replicable and viable inthe relevant host can be used.

Vectors can be employed to transform an appropriate host to permit thehost to express an inventive protein or polypeptide. Examples ofappropriate expression hosts include: bacterial cells, such as E. coli,B. subtilis, Streptomyces, and Salmonella typhimurium; fungal cells,such as Saccharomyces cerevisiae, Pichia pastoris, and Neurosporacrassa; insect cells such as Drosophila and Spodoptera frugiperda;mammalian cells such as CHO, COS, BHK, HEK 293 br Bowes melanoma; plantcells e.g., Nicotiana tabacum, a dicot plant species, or corn, a monocotplant species; algal cells e.g., Chlamydomonas reinhardtii; or explantsof any plant tissues, e.g., leaf, stem or root segments, etc.

In bacterial systems, a number of expression vectors may be selecteddepending upon the use intended for the triterpene synthase polypeptide.For example, when large quantities of triterpene synthase polypeptide orfragments thereof are needed for commercial production or for inductionof antibodies, vectors which direct high level expression of fusionproteins that are readily purified can be desirable. Such vectorsinclude, but are not limited to, multifunctional E. coli cloning andexpression vectors such as BLUESCRIPT™ (Stratagene), in which thetriterpene synthase polypeptide coding sequence may be ligated into thevector in-frame with sequences for the amino-terminal Met and thesubsequent 7 residues of 6-galactosidase so that a hybrid protein isproduced; pIN vectors (Van Heeke & Schuster (1989) J. Biol. Chem. 264:5503-5509); pET vectors (Novagen, Madison Wis.); and the like.

Similarly, in the yeast Saccharomyces cerevisiae a number of vectorscontaining constitutive or inducible promoters isolated from, e.g., analpha factor, an alcohol dehydrogenase or a PGH gene may be used forproduction of the triterpene synthase polypeptides of the invention. Forreviews, see Ausubel (supra) and Grant et al. (1987) Methods inEnzymology 153:516-544 (incorporated herein by reference).

Plant and algal systems may also be used for expression of triterpenesynthase. Transcription of sequences encoding triterpene synthase may bedriven by viral promoters, e.g., the 355 and 19S promoters of CaMV usedalone or in combination with the omega leader sequence from TMV(Takamatsu, N. (1987) EMBO J. 6:307-311). Alternatively, plant promoterssuch as, e.g., the small subunit of RUBISCO or heat shock promoters maybe used. (See, e.g., Coruzzi, G. et al. (1984) EMBO J. 3:1671-1680;Broglie, R. et al. (1984) Science 224:838-843; and Winter, J. et al.(1991) Results Probl. Cell Differ. 17:85-105.) For algal expressionwork, a strong constitutive promoter includes, e.g., a □-tubulin genepromoter (see Brunke, K J et al. (1984) Molec. Cell. Biol. 4:1115-1124). These constructs can be introduced into plant cells, forexample, by direct DNA transformation or pathogen-mediated transfection.(See, e.g., The McGraw Bill Yearbook of Science and Technology (1992)McGraw Hill, New York N.Y., pp. 191-196.)

Also provided are engineered host cells that are transduced (transformedor transfected) with a vector provided herein (e.g., a cloning vector oran expression vector), as well as the production of polypeptides of theinvention by recombinant techniques. The vector may be, for example, aplasmid, a viral particle, a phage, etc. The engineered host cells canbe cultured in conventional nutrient media modified as appropriate foractivating promoters, selecting transformants, or amplifying thetriterpene synthase gene. Culture conditions, such as temperature, pHand the like, are those previously used with the host cell selected forexpression, and will be apparent to those skilled in the art and in thereferences cited herein, including, e.g., Sambrook, Ausubel and Berger,as well as e.g., Freshney (1994) Culture of Animal Cells: A Manual ofBasic Technique, 3rd ed. (Wiley-Liss, New York) and the references citedtherein.

In other embodiments, methods for producing a cell that catalyzes aunique chemical condensation of 2 FPP molecules creating a branched,triterpene hydrocarbon, botryococcene, are provided. Such methodsgenerally include: (a) transforming a host cell with an isolated nucleicacid molecule encoding a triterpene synthase polypeptide, e.g., anucleic acid molecule encoding a polypeptide comprising SEQ ID NO: 2, avariant, preferably a conservative variant, of SEQ ID NO: 2, or afragment of SEQ ID NO: 2 having triterpene synthase activity and (b)culturing the transformed cell to produce the botryococcene. For examplea transformed yeast cell of this invention may be cultured by largescale fermentation, providing the added advantage of producing largeamounts of triterpenes, particularly botryococcene.

In other embodiments, methods for selecting a cell that converts 2 FPPto botryococcene are provided. The methods generally include: (a)providing a host cell containing a nucleic acid construct that includesa nucleotide sequence that encodes a triterpene synthase polypeptide.The methods further include (b) culturing the cell in the presence of asuitable 2 FPP and under conditions where the triterpene synthase isexpressed at an effective level; and (c) detecting the production ofbotryococcene.

In other embodiments, methods for producing botryococcene are provided.In one aspect, the methods for producing a botryococcene compriseculturing a host cell transfected with a nucleic acid molecule thatencodes a triterpene synthase, preferably a triterpene synthase of thisinvention under conditions sufficient for production of a botryococcene.Optionally, the botryococcene produced by the host cells are isolated.The host cell may be, for example, a cell in culture, e.g., the yeaststrain TN7 transfected with a vector of this invention, or it may be acell which is part of an organism such as a transfected cell in aterrestrial plant. In addition to transfection with triterpenesynthase-encoding nucleic acid molecule, such plant cells may also becotransfected with nucleic acid molecules encoding for one or more otherenzymes in the triterpene synthesis pathway, such as the genes forfarnesyl diphosphate synthase or a triterpene synthase such as squalenesynthase or triterpene methyltransferase. Plant cells for transfectioninclude, for example algal cells such as Botryococcus spp. cells (e.g.,Botryococcus braunii), Chlamydomonas spp. cells or terrestrial plantcells, such as a tobacco plant cell. Transfection of plant cells withexogenous genes may be directed to the cytosolic compartment, thechloroplast or both. In other embodiments, cells other than plant cellsmay be transformed with triterpene synthase-encoding nucleic acidmolecules, and optionally with nucleic acid molecules encoding one ormore other enzymes involved in triterpene synthesis. These cellsinclude, for example, prokaryotic cells such as bacteria and eukaryoticcells, such as fungi or animal cells. In particular the cells may be anatural or recombinant yeast cells, e.g., yeast cells that accumulateFPP but do not metabolize squalene, e.g., yeast cells with a mutant ordeleted or disrupted EGR1 gene such that it produces reduced or nosqualene epoxidase, e.g., a yeast strain such as TN7. In any of theaforementioned embodiments, the cells may also be genetically altered toenhance the production of farnesyl diphosphate and thereby provide alarger precursor pool for triterpene synthesis, such as through geneknockout, so as to eliminate or reduce diversion of farnesyl diphosphatefor use in synthesis of metabolites other than triterpenes, such assesquiterpenes, sterols, or polyprenols, or to eliminate or reduce theaction of phosphatase(s) on farnesyl diphosphate. The production oftriterpenes may also be enhanced by diverting other metabolicintermediates such as, e.g., isopentenyl diphosphate or dimethylallyldiphosphate (DMAPP) to the production of FPP, therein providing enhancedcarbon flux to a key intermediate for the biosynthesis of triterpenes.

Also an aspect of this invention is a method for producing triterpenescomprising transfecting a yeast strain having high intracellularconcentrations of FPP and reduced levels of sqalene epoxidase with anucleic acid molecule encoding a triterpene synthase and culturing thetransfected cells under conditions suitable for the production oftriterpenes. The triterpene synthase may be, e.g., a botryococcenesynthase or a squalene synthase. The botryococcene synthase may be,e.g., a botryococcene synthase of this invention, e.g., a botryococcenesynthase comprising the amino acid sequence of SEQ ID NO:2 or aconservative variant thereof, or a fragment thereof having botryococcenesynthase activity. The high intracellular concentrations of FPP may be,e.g, at least 10 mg/L, at least 20 mg/L, at least 30 mg/L, at least 40mg/L, at least 50 mg/L, at least 60 mg/L, at least 70 mg/L, or at least80 mg/L. The reduced squalene epoxidase may be, e.g., less than thelevels of squalene epoxidase found in the yeast strain CALI-7 (Takahashiet al., (2007) “Metabolic Engineering of Sesquiterpene Metabolism inYeast” Biotech. Bioeng. 170-181). The reduced squalene expoxidase levelsmay also be undectable levels, such as the levels in the yeast strainTN7.

In another aspect, cells transfected with a nucleic acid moleculeencoding a triterpene synthase are cultured under conditions suitablefor the expression of the triterpene synthase polypeptide and an extractrich in triterpene synthase is then prepared. This extract may be, forexample, a cell paste or tissue homogenate, or it may be, for example, apurified or partially purified preparation of triterpene synthase. FPP,e.g., radiolabelled FPP, plus or minus reducing equivalents (NaDPH) andalgal lysates is then exposed to the extract rich in triterpene synthaseunder conditions which allow for production of botryococcene. Thereductive condensation may be via a batch process or a continuousprocess. Optionally the botryococcene may then be isolated.

As previously discussed, general texts which describe molecularbiological techniques useful herein, including the use of vectors,promoters and many other relevant topics, include Berger and Kimmel,Guide to Molecular Cloning Techniques, Methods in Enzymology Volume 152,(Academic Press, Inc., San Diego, Calif.) (“Berger”); Sambrook et al.,Molecular Cloning—A Laboratory Manual, 2d ed., Vol. 1-3, Cold SpringHarbor Laboratory, Cold Spring Harbor, N.Y., 1989 (“Sambrook”) andCurrent Protocols in Molecular Biology, F. M. Ausubel et al., eds.,Current Protocols, a joint venture between Greene Publishing Associates,Inc. and John Wiley & Sons, Inc., (supplemented through 1999)(“Ausubel”) (each of which is incorporated by reference). Examples ofprotocols sufficient to direct persons of skill through in vitroamplification methods, including the polymerase chain reaction (PCR),the ligase chain reaction (LCR), Qβ-replicase amplification and otherRNA polymerase mediated techniques (e.g., NASBA), e.g., for theproduction of the homologous nucleic acids of the invention are found inBerger, Sambrook, and Ausubel, as well as in Mullis et al. (1987) U.S.Pat. No. 4,683,202; Innis et al., eds. (1990) PCR Protocols: A Guide toMethods and Applications (Academic Press Inc. San Diego, Calif.)(“Innis”); Arnheim & Levinson (Oct. 1, 1990) C&EN 36-47; The Journal OfNIH Research (1991) 3: 81-94; Kwoh et al. (1989) Proc. Natl. Acad. Sci.USA 86: 1173; Guatelli et al. (1990) Proc. Nat'l. Acad. Sci. USA 87:1874; Lomell et al. (1989) J. Clin. Chem. 35: 1826; Landegren et al.(1988) Science 241: 1077-1080; Van Brunt (1990) Biotechnology 8:291-294; Wu and Wallace (1989) Gene 4:560; Barringer et al. (1990) Gene89:117; and Sooknanan and Malek (1995) Biotechnology 13: 563-564 (eachof which is incorporated by reference). Improved methods for cloning invitro amplified nucleic acids are described in Wallace et al., U.S. Pat.No. 5,426,039. Improved methods for amplifying large nucleic acids byPCR are summarized in Cheng et al. (1994) Nature 369: 684-685 and thereferences cited therein (incorporated by reference herein), in whichPCR amplicons of up to 40 kb are generated. One of skill will appreciatethat essentially any RNA can be converted into a double stranded DNAsuitable for restriction digestion, PCR expansion and sequencing usingreverse transcriptase and a polymerase. See, e.g., Ausubel, Sambrook andBerger, all supra.

These and other embodiments of the present invention will readily occurto those of ordinary skill in the art in view of the disclosure herein,and are specifically contemplated.

The invention is further understood by reference to the examples setforth herein, which are intended to be purely exemplary of theinvention. The present invention is not limited in scope by theexemplified embodiments, which are intended as illustrations of singleaspects of the invention only. Any methods that are functionallyequivalent are within the scope of the invention. Various modificationsof the invention in addition to those described herein will becomeapparent to those skilled in the art from the foregoing description.Such modifications fall within the scope of the appended claims.

EXAMPLES Example 1 Isolation of the Botryococcus braunii BotryococceneSynthase Gene

A cDNA library was constructed from mRNA isolated from cells in rapidgrowth phase, corresponding to 9 days after subculturing, converted todouble stranded cDNAs, and the cDNAs inserted into the lambda ZAP™vector (Stratagene, La Jolla, Calif.) as previously described by Okadaet al. (2000) “Molecular characterization of squalene synthase from thegreen microalga Botryococcus braunii, race B”. Archives Of Biochemistryand Biophysics 307-317. Plaque lifts of the cDNA library were preparedand hybridized with an 32PdCTP-radiolabled full-length squalene synthasecDNA probe (referred to as SS1386 because it is 1386 by long) using thePrime-It™ kit (Stratagene) at 30° C. in hybridization buffer consistingof 5×SSPE, 2×Denhardt's solution, 0.2% SDS, 100 g/ml salmon sperm DNAand 40% formamide, also according to Okada et al. (2000). The plaquelifts were then washed three times at room temperature for 5 min with2×SSC, 0.1% SDS and hybridization detected by autoradiography. After 2rounds of plaque purification, isolated plaques were converted to theirplasmid forms following the procedures recommended by the manufacturer(Stratagene), and restriction digestions of the isolated plasmidscompared. Those plasmids exhibiting restriction patterns similar to thatfor the squalene synthase were discarded and only those showingdistinctive differences examined further by automated DNA sequencing(ABI 310 genetic analyzer, PE applied Biosystems, Foster City, Calif.).

DNA sequence of the entire botryococcene synthase (BBS) cDNA clone (FIG.4) yielded a putative full-length cDNA clone coding for a 402 amino acidprotein having a predicted molecular size of 45,692 daltons as shown inFIG. 5. Alignment of the predicted amino acid sequence from the BBS cDNAto other well described squalene synthase proteins from plants, animalsand microbes demonstrated that there were several highly conserveddomains shared between the predicted BBS protein and the varioussqualene synthases, but also there were several regions highly conservedamongst the various squalene synthases not found in the BBS protein(FIG. 6). Several of the domains conserved between BBS and the squalenesynthases have been associated with early steps in the squalene synthaseenzymatic reactions (domains I-IV), while domain V, associated with theconversion of PSPP to squalene, and a membrane-spanning domain (domainVI) are not conserved in the BBS protein.

Example 2 Functional Characterization of the Botryococcene SynthaseEnzyme

The entire open reading frame, ORF, region of the BBS cDNA was amplifiedusing standard RT-PCR conditions with a forward primer (5′CCCGCCACACAGCAGACTTTCAGGG 3′ SEQ ID NO: 38, a reverse primer (5′CCTGGATGACATGACCAGCCGTGC 3′ SEQ ID NO: 39 (designed according to the DNAsequence obtained from the initial BBS cDNA isolated from the cDNAlibrary) and first strand cDNA as template. First strand cDNA wasprepared from RNA isolated from rapidly growing cells via the Triazolmethod according the manufacturer's instructions (Invitrogen, Carlsbad,Calif.), and converted to single-strand DNA using oligo-dT primer andreverse transcriptase (Okada et al. 2000). The PCR amplification productwas subsequently cloned in the pGEM T-Easy vector (Promega, Madison,Wis.) and the resulting recombinant plasmid subjected to automated DNAsequencing. The BBS cDNA was then PCR amplified from the pGEM vectorusing various primer combinations for the insertion of the BBS cDNA intobacterial expression vectors and into yeast expression vectors. Tocreate bacterial expression vectors, a forward-primer (5′TTGGCGCCTATGACTATGCACCAAGACCACGG SEQ ID NO: 40) harboring an AscIrestriction site reverse—(in bold) and primer (5′GGGGGCGCCTCACTTGGTGGGAGTTGGGGCTGCGC SEQ ID NO: 41) containing an XhoIrestriction site (in bold) to PCR amplify the intact BBS cDNA from thepGEM vector and the amplified DNA ligated into the AscI and XhoI sitesof a modified pET28b vector (Novagen, Madison Wis.) This vector wasmodified to contain an AscI restriction site 3′ to the transcriptionalelements within the vector. In order to generate a BBS protein with ahexa-histidine (SEQ ID NO: 44) amino-terminal extension, the full-lengthBBS cDNA was released from the pET28b vector by digestion with BamHI andXhoI, and the isolated fragment ligated into the corresponding sites ofthe pET28a vector. The pET28a expression vector is designed to include aDNA sequence coding for a hexa-histidine (SEQ ID NO: 44) amino acidextension fused in-frame with the amino terminus of the BBS cDNA,creating a fusion protein that facilitates nickel affinity purificationof the bacterial expressed BBS protein The recombinant pET28a vector wastransformed into E. coli strain BL21(DE3) according to themanufacturer's recommendations (Novagen) and the engineered bacteriawere selected for growth in the presence of a suitable antibioticselection marker.

E. coli harboring the recombinant plasmid were grown in liquid LB brothat 37° C. with vigorous shaking until the cultures reached an opticaldensity of ˜0.8 (OD nm), then expression of the BBS cDNA was induced byaddition of 0.1 mM isopropylthio-B-D-galactoside (IPTG) and the culturesallowed to incubate for an additional 5 to 20 hours with shaking at roomtemperature. One hundred ml of the culture were subsequently collectedby centrifugation at 4,000° g. for 10 min, resuspended in 10 ml of lysisbuffer (50 mM Tris-HCl, 2 mM MgCl₂ mM β-mercaptoethanol, 1 mM EDTA, 5%(v/v) glycerol, pH 7.5), then sonicated 5 times for 20 seconds with amicroprobe sonicator at 60% maximum power. The samples were cooled onice for 2 min between sonication treatments. The sonicate wascentrifuged at 16,000 g for 15 min at 4° C. and used either forpurification of the hexa-histidine tagged (SEQ ID NO: 44) BBS enzyme or5-20 μl of the supernatant (corresponding to 10-100 g of total solubleprotein) used for the botryococcene synthase enzyme assays as describedby Okada et al., (2004) Arch. Biochem. Biophys. 110-118.

Purification of the bacterial expressed BBS enzyme was afforded by theamino-terminal hexa-histidine tag (SEQ ID NO: 44) using standard nickelaffinity chromatography. In brief, aliquots of the bacterial lysate wereapplied to activated nickel columns according the manufacturer'srecommendations (Novagen) and non-associating proteins eluted from thecolumn using wash buffer. Selective elution of the hexa-histidine (SEQID NO: 44) BBS enzyme was observed at increasing concentrations of thecounter ion, imidazole, with maximum recovery of protein at 250 mMimidazole and recovery of protein with the expected molecular size of49,000 daltons (FIG. 7). Lysate prepared from bacteria over-expressingthe BBS cDNA was prepared, applied to a nickel affinity column, and thenproteins eluted with increasing concentrations of imidazole. Aliquots ofthe eluded fractions were examined by SDS-PAGE and stained withCoommassie Blue. Bradford dye analysis indicated that the proteincontent in the 250 mM imidazole fraction was 0.2 mg/ml.

Typical BBS enzyme assays were initiated by mixing aliquots of 5 μl ofE. coli lysate or purified BBS enzyme with 50 mM Tris, pH 7.0, mM MgCl 5mM-mercaptoethanol 4 M [3^(H)]-FPP (1,800, dpm/pmole), plus as indicated2 mM NADPH and 5 μl of lysate prepared from cells according to Okada etal. (2004) in 50 l total volume. Reactions were incubated at 37° C. for1 h, then extracted with 100 ul hexane. Forty μl of the hexane extractwas then spotted onto silica TLC plates with authentic standards ofbotryococcene and squalene, developed with hexane, and the standardsvisualized with iodine vapors. The TLC zones corresponding to squaleneand botryococcene were scrapped and analyzed by scintillationspectrometry (see assay illustration, FIG. 8). After incubating lysatesor purified BBS enzyme prepared from E. coli over-expressing the BBScDNA with radiolabeled FPP, plus and minus reducing equivalents (NADPH)and algal lysates, the reaction products were separated by TLC prior todetermining the amount of radioactivity incorporated in squalene(control) and botryococcene.

Botryococcus lysate was prepared as described by Okada et al., (2004)and enzyme assays were performed with the components indicated in FIG.9, then incubated at 37° C. for 1 h, and reaction products were thenextracted with hexane. Aliquots of the hexane extracts was separated bysilica TLC and the radioactivity migrating to zones corresponding toauthentic standards of botryococcene and squalene determined byscintillation spectrometry. As shown in FIG. 9, incubation of thepurified BBS enzyme with FPP, plus or minus the inclusion of NADPH,resulted in little, if any, botryococcene being formed. Similar lowlevels of background activity were observed in control incubationswithout any BBS enzyme being added. Botryococcene biosynthesis wasevident at a very low level in incubations of the lysate, as wasreported by Okada et al. (2004) earlier, but was dependent upon theaddition of NADPH to the reaction mixture. However, a 5 to 10 foldstimulation of botryococcene biosynthesis was observed when the purifiedBBS enzyme was incubated with the lysate (FIG. 9). This botryococcenesynthase activity was dependent upon the addition of NADPH, was equallysupported by the additional of NADH, was time dependent with maximalactivity observed after 1 hr incubation, and suggested that the BBSrequired an accessory or complementary factor found in the algal lysatefor full enzyme activity.

Additional experiments demonstrated that the BBS enzyme was essential tothe formation of botryococcene in these assays. Incubation of thepurified BBS enzyme at 95° C. for 5 min prior to the enzyme assayseliminated all apparent botryococcene biosynthesis. Pre-treatment of thelysate with proteinase K, likewise abolished the botryococcenebiosynthetic activity.

Altogether, the experimental data suggest that the BBS enzyme was notsufficient for botryococcene biosynthesis by itself and that anadditional partner factor, perhaps an accessory protein (as suggested bythe proteinase K sensitivity) as provided by the algal lysate, wasnecessary to observe the full complement of enzyme activity. Additionalevidence for this suggestion was provided by over-expression of the BBSgene in yeast.

Example 3 Over Expression of the BBS Enzyme in Yeast

A yeast line, CALI-7, generates high intracellular concentrations ofFPP. (Takahashi et al., (2007) “Biotech. Bioeng. 170-181). One furthermodification was introduced into this yeast line i.e., an insertionalmutation in the ERG 1 gene. The ERG 1 gene of yeast encodes for theenzyme squalene epoxidase (Andrositz et al. (1991) Gene 155-60), whichconverts squalene to an epoxide form. The insertional mutation of thisgene was created by introducing the TRP1 gene flanked by DNA sequencesof the 5′ and 3′ region of the ERG 1 gene into the CALI-7 cells andsubsequent selection for tryptophan auxotrophic growth according to themethod of Wang et al. (2004) Methods 199-205. This modified yeast lineis capable of accumulating high levels of FPP (Song, (2003) Anal.Biochem. 180-1 85), but not metabolizing squalene was denoted as TN7.

The BBS gene was then inserted into a standard yeast expression vectorpYEP352 harboring an ADH1 promoter (Takahashi et al., 2007). This wasaccomplished by PCR amplification of the BBS gene with oligo nucleotideprimers (5′ CCGGAATTCAAAACAATGACTATGCACCAAGA CCACGG SEQ ID No. 42, EcoR1restriction site in bold, 5′ CCCAAGCTTCACTTGGTGGGAGTTGGGGCTGCGC SEQ IDNo. 43, HindIII restriction site in bold) that introduced uniquerestriction enzyme sites at the 5′ and 3′ ends of the amplificationproduct, digestion of the PCR amplification product with EcoR1 andHindIII restriction enzymes, followed by ligation of the isolated BBSDNA fragment into the pYEP352 vector digested with correspondingenzymes. The recombinant yeast expression vector was designated aspYEP-BBS and was introduced into the TN7 yeast line via lithium acetatetransformation (Takahashi et al., 2007), followed by selection foruracil auoxtrophic growth. A yeast line confirmed to possess thepYEP-BBS expression vector was identified by colony PCR and designatedas TN7-BBS.

Individual colonies of TN7 and TN7-BBS were subsequently grown in 25 mlof YPDE media for 8 days at room temperature before analyzing thecultures for production of novel triterpene components (FIG. 10) Inbrief, 1 ml aliquots of the culture were combined with 1 ml of acetone,vigorously mixed, and incubated at room temperature for 3 min. One ml ofhexane was added and mixed vigorously for 60 seconds. The mixture wasthen centrifuged briefly at 100 g to separate the phases, and theorganic phase removed and concentrated to dryness under a nitrogenstream. The dried extract was resuspended in 50 μl of hexane and a 1 μlaliquot injected into a Thermo-Finnigan GC-MS. Compounds were separatedon a Restek Rtx-5 (30 m by 0.25 μm) column with a initial temperature of200° C. for 1 min, followed by an increase to 280° C. at 4° C./min, thento 320° C. with a 20° C./min ramp and a final 5 min hold at 320° C. Massspectra were recorded in a DSQ quadrapole with the ionization set at 70eV.

Comparison of the compounds accumulating specifically in the TN7-BBSlines and not in the TN7 lines identified pre-squalene alcohol, thede-phosphorylated product of PSPP, as the only unique compoundcorrelated with expression of the BBS gene (FIG. 10). This is consistentwith the observation that additional factors provided by the lysate arenecessary for the BBS enzyme to convert FPP to PSPP and then onto to thefinal product of botryococcene.

Example 4 Accumulation of Squalene in TN7 Yeast Over-Expressing a B.Braunii Squalene Synthase Gene

A yeast TN7 harboring the expression vector YEpD60 containing a squalenesynthase gene was produced by transfecting TN7 with DNA sequenceencoding the B. braunii squalene synthase gene as described by Okada etal. (2002). Recombinant yeast, verified by colony PCR for the plasmid,were grown in defined media and aliquots of the cell culture collectedat the indicated times. For squalene determination, equal volumes ofacetone were added to the cell culture samples, vortexed, incubated 15min, then exacted with 2 volumes of hexane. The collected hexane waspassed over a silica column and flow-through analyzed by GC as describedabove. The accumulation of squalene in TN7 yeast is shown in FIGS. 11and 12.

1. An isolated polypeptide having triterpene synthase activity whereinthe polypeptide comprises a peptide domain selected from domains I, II,III, IV, and V, wherein: domain I is at least 65% homologous toLPQELQDPICIFYL (SEQ ID NO: 3.), domain II is at least 65% homologous toLRALDTVEDDMN LKSETK (SEQ ID NO; 4), domain III is at least 50%homologous to YCHYVAGSCGIAV TKVIV (SEQ ID NO: 5), domain IV is at least80% homologous to GLLLQKANIITD YNED (SEQ ID NO 6), domain V is at least40% homologous to GLLLQKANIITD YNED ALALLLVTAFGHLS (SEQ ID NO: 7), and2. The isolated polypeptide of claim 1, comprising domains I, II, III,IV, and V wherein, (a) domain I comprises an amino acid sequence that isat least 65% or at least 70% identical the full-length of SEQ ID NO: 3,(b) domain II comprises an amino acid sequence that is at least 65% orat least 70% identical the full-length of SEQ ID NO: 4, (c) domain IIIcomprises an amino acid sequence that is at least 65% or at least 70%identical the full-length of SEQ ID NO: 5, (d) domain IV comprises anamino acid sequence that is at least 80% or at least 85% identical thefull-length of SEQ ID NO: 6 and (e) domain V comprises an amino acidsequence that is at least 40% or at least 50% identical the full-lengthof SEQ ID NO:
 7. 3. The isolated polypeptide of claim 1, wherein domainI comprises SEQ ID NO: 3, domain II comprises SEQ ID NO: 4, domain IIIcomprises SEQ ID NO: 4, domain IV comprises SEQ ID NO: 6 and domain Vcomprises SEQ ID NO:
 7. 4. The polypeptide of claim 1 which is aBotryococcus braunii triterpene synthase.
 5. The polypeptide of claim 1which comprises the amino acid sequence of SEQ ID NO:2.
 6. Thepolypeptide of claim 1 which comprises at least 80% sequence identity tothe amino acid sequence of SEQ ID NO:2.
 7. An isolated nucleic acid,which encodes the polypeptide of claim
 1. 8. An isolated nucleic acid,which encodes the polypeptide of claim
 3. 9. A vector comprising thenucleic acid of claim
 5. 10. The vector of claim 9 wherein the nucleicacid molecule encodes a polypeptide comprising the amino acid sequenceset forth in SEQ ID NO:2.
 11. A host cell transfected with the nucleicacid molecule of claim
 7. 12. A host cell transfected with the vector ofclaim
 9. 13. The host cell of claim 12 wherein the cell is procaryoticor eukaryotic cell.
 14. The host cell of claim 13 wherein theprocaryotic cell is a bacterial cell.
 15. The host cell of claim 13wherein the eucaryotic cell is a fungal cell, plant cell or animal cell.16. The host cell of claim 15, where in the plant cell is a transfectedterrestrial plant cell or algae cell.
 17. The host cell of claim 13,wherein the eukaryotic cell is a yeast cell that accumulates FPP. 18.The host cell of claim 17 wherein the cell is yeast strain TN7.
 19. Thehost cell of claim 16 further transfected with a nucleic acid moleculethat encodes a farnesyl diphosphate synthase, a triterpenemethyltransferase, a squalene methyltransferease or a botryococcenemethyltrasferase.
 20. The host cell of claim 16 further transfected witha nucleic acid molecule that encodes a farnesyl diphosphate synthase anda nucleic acid molecule that encodes a triterpene methyltransferase, andwherein the nucleic acid molecules encoding the triterpene synthase,farnesyl diphosphate synthase, and triterpene methyltransferase are alltargeted for expression in a chloroplast.
 21. The host cell of claim 17wherein the yeast cell comprises (i) an ERG1 gene knockout such that theyeast cell does not metabolize squalene, or (ii) a mutated ERG1 genesuch that squalene epoxidase is synthesized at reduced levels and theyeast cell metabolizes squalene at reduced levels.
 22. A method forproducing triterpenes comprising transfecting a yeast cell having highintracellular concentrations of FPP and reduced levels of squaleneepoxidase with a nucleic acid molecule encoding a triterpene synthaseand culturing the transfected yeast cell under conditions suitable forproduction of triterpenes.
 23. The method of claim 22 wherein thetriterpene synthase is a botryococcene synthase or a squalene synthase.24. The method of claim 22 further comprising isolating triterpene fromthe cultured yeast cells.
 25. The method of claim 23 wherein the yeastcells are cultured under large scale fermentation conditions.